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Implementation of a siRNA Screen for Prostate Tumour Cell Lines Using Living Cell Arrays

Title:
Implementation of a siRNA Screen for Prostate Tumour Cell Lines Using Living Cell Arrays
Abstract:
Prostate cancer is one of the most common causes of death in men. In this work a siRNA screen of around 1500 cancer-relevant genes was performed using 3 different cell lines (VCaP, LAPC-4, RWPE-1). A novel technique, the living cell array, was initiated in order to obtain information about the biology of Androgeninduced growth in prostate tumour cell lines. This technique is based on the principal of reverse transfection [1] and genes are knocked down by siRNAs. The cells on the living cell array were set under stress by reduction of the androgens in the media while the proliferation and apoptosis were quantified. The statistical analysis of the data implicates the success of the screen and shows that this method is suitable for large-scale experiments.
Year:
2008
Publication type:
Journal article
Journal:
Wissenschaftliche Beiträge 2008
ISSN:
0949-8214
Volume:
13
Pages:
19-24
Language:
English
Document status:
Open access
PubListerURL:
https://publister.bib.th-wildau.de/publister/public/publication/1049
Reich, P., Frohme, M., Rantala, J., & Nees, M. (2008). Implementation of a siRNA Screen for Prostate Tumour Cell Lines Using Living Cell Arrays Wissenschaftliche Beiträge 2008. 13, 19-24.
@article{1049,
    author           = {Reich, Peggy and Frohme, Marcus and Rantala, Juha and Nees, Matthias},
    title            = {Implementation of a siRNA Screen for Prostate Tumour Cell Lines Using Living Cell Arrays},
    journal          = {Wissenschaftliche Beiträge 2008},
    year             = {2008},
    volume           = {13},
    pages            = {19-24},
    doi              = {http://dx.doi.org/10.15771/0949-8214_2008_1_3},
    url              = {http://www.th-wildau.de/fileadmin/dokumente/forschung_transfer/dokumente_offen/wissbeitraege/WB_2008.pdf},
}


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