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Miniaturized Flow-Through Bioreactor for Processing and Testing in Pharmacology

Title:
Miniaturized Flow-Through Bioreactor for Processing and Testing in Pharmacology
Abstract:
Conventional Bioreactor systems for cultivating cells in Life Science have been widely used for decades. An in vitro cell cultivation bioreactor should reliably and reproducibly mimic the in vivo microenvironment of the cultured cells. Normally, mammalian cell cultures are performed in conventional bioreactor devices such as culture flasks and culture-dishes. However, these tools have fundamental limitations due to being inappropriate for high throughput screening and consume a considerable amount of resources and time [1]. Therefore, there is a trend towards miniaturization, disposables and even micro platforms that fulfill increasing demands strongly aiming for production and testing of novel pharmaceutical products. Here we present the development and manufacture of a disposable miniaturized flow-through bioreactor system that can be produced in large numbers at low costs. nanoporous hollow fibers are located at the fluidic sources and drains of the miniaturized bioreactors and retain cells. The necessary mixture of oxygen and carbon dioxide is provided via diffusion through a semi-permeable membrane. Fluidic connections allow the continuous feeding of the cells adding nutrient solution at constant rates at the inlet of the micro bioreactor and removing the solution at the same rate at the outlet. This medium can be collected and used for subsequent analysis. Different designs and concepts for such bioreactors were carried out with varying numbers of plates, and integrated or joined miniaturized reactor chambers. First tests show full technical and biological functionality, cells could successfully be cultivated at high viability rates for some days.
Year:
2016
Publication type:
Conference contribution
Conference:
THERMEC 2016
Book title:
THERMEC 2016 : selected, peer reviewed papers from the 9th International Conference on Processing & Manufacturing of Advanced Materials
Series:
Materials Science Forum (Volume 879)
Pages:
236-243
Language:
English
Document status:
Published
PubListerURL:
https://publister.bib.th-wildau.de/publister/public/publication/2022
Keywords:
Bioreactor, Miniaturization, Perfusion, Pharmacology, Tightness Testing
Böhme, A., Radke, L., Schütze, F., Schneider, S., Liebscher, T., Sauer, S., et al. (2016). Miniaturized Flow-Through Bioreactor for Processing and Testing in Pharmacology THERMEC 2016 : selected, peer reviewed papers from the 9th International Conference on Processing & Manufacturing of Advanced Materials (pp. 236-243). (Materials Science Forum (Volume 879)).
@inproceedings{2022,
    author           = {Böhme, Andrea and Radke, Lars and Schütze, Felix and Schneider, Sylvio and Liebscher, Thilo and Sauer, Sabine and Santo, Loredana and Quadrini, Fabrizio and Hummel, Michael and Giese, Christoph and Frohme, Marcus and Foitzik, Andreas},
    title            = {Miniaturized Flow-Through Bioreactor for Processing and Testing in Pharmacology},
    year             = {2016},
    pages            = {236-243},
    series           = {Materials Science Forum (Volume 879)},
    booktitle        = {THERMEC 2016 : selected, peer reviewed papers from the 9th International Conference on Processing & Manufacturing of Advanced Materials},
    doi              = {10.4028/www.scientific.net/MSF.879.236},
}


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