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Alizarin Red S for Online Pyrophosphate Detection Identified by a Rapid Screening Method

Title:
Alizarin Red S for Online Pyrophosphate Detection Identified by a Rapid Screening Method
Abstract:
We identified Alizarin Red S and other well known fluorescent dyes useful for the online detection of pyrophosphate in enzymatic assays, including the loop mediated isothermal amplification (LAMP) and polymerase chain reaction (PCR) assays. An iterative screening was used for a selected set of compounds to first secure enzyme compatibility, evaluate inorganic pyrophosphate sensitivity in the presence of manganese as quencher and optimize conditions for an online detection. Of the selected dyes, the inexpensive alizarin red S was found to selectively detect pyrophosphate under LAMP and PCR conditions and is superior with respect to its defined red-shifted spectrum, long shelf life and low toxicity. In addition, the newly identified properties may also be useful in other enzymatic assays which do not generate nucleic acids but are based on inorganic pyrophosphate. Finally, we propose that our screening method may provide a blueprint for rapid screening of compounds for detecting inorganic pyrophosphate.
Year:
2017
Publication type:
Journal article
Journal:
Scientific Reports
ISSN:
2045-2322
Volume:
7
Pages:
45085
Language:
English
Document status:
Open access
PubListerURL:
https://publister.bib.th-wildau.de/publister/public/publication/2217
Fischbach, J., Loh, Q., Bier, F., Lim, T., Frohme, M., & Glökler, J. (2017). Alizarin Red S for Online Pyrophosphate Detection Identified by a Rapid Screening Method Scientific Reports. 7, 45085.
@article{2217,
    author           = {Fischbach, Jens and Loh, Qiuting and Bier, Frank F. and Lim, Theam Soon and Frohme, Marcus and Glökler, Jörn Felix},
    title            = {Alizarin Red S for Online Pyrophosphate Detection Identified by a Rapid Screening Method},
    journal          = {Scientific Reports},
    year             = {2017},
    volume           = {7},
    pages            = {45085 },
    publisher        = {Springer Nature},
    doi              = {10.1038/srep45085},
}


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