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Engineering of CHO Cells for the Production of Recombinant Glycoprotein Vaccines with Xylosylated N-glycans

Title:
Engineering of CHO Cells for the Production of Recombinant Glycoprotein Vaccines with Xylosylated N-glycans
Abstract:
Xylose is a general component of O-glycans in mammals. Core-xylosylation of N-glycans is only found in plants and helminth. Consequently, xylosylated N-glycans cause immunological response in humans. We have used the F-protein of the human respiratory syncytial virus (RSV), one of the main causes of respiratory tract infection in infants and elderly, as a model protein for vaccination. The RSV-F protein was expressed in CHO-DG44 cells, which were further modified by co-expression of β1,2-xylosyltransferase from Nicotiana tabacum. Xylosylation of RSV-F N-glycans was shown by monosaccharide analysis and MALDI-TOF mass spectrometry. In immunogenic studies with a human artificial lymph node model, the engineered RSV-F protein revealed improved vaccination efficacy.
Year:
2017
Publication type:
Journal article
Journal:
Bioengineering
ISSN:
2306-5354
Number:
2
Volume:
4
Pages:
1-12
Language:
English
Document status:
Open access
PubListerURL:
https://publister.bib.th-wildau.de/publister/public/publication/2251
Keywords:
CHO; glycoengineering; respiratory syncytial virus; vaccine; xylose
Sandig, G., von Horsten, H., Radke, L., Blanchard, V., Frohme, M., Giese, C., et al. (2017). Engineering of CHO Cells for the Production of Recombinant Glycoprotein Vaccines with Xylosylated N-glycans Bioengineering. 4 (2), 1-12.
@article{2251,
    author           = {Sandig, Grit and von Horsten, Hans Henning and Radke, Lars and Blanchard, Véronique and Frohme, Marcus and Giese, Christoph and Sandig, Volker and Hinderlich, Stephan},
    title            = {Engineering of CHO Cells for the Production of Recombinant Glycoprotein Vaccines with Xylosylated N-glycans},
    journal          = {Bioengineering},
    year             = {2017},
    volume           = {4},
    number           = {2},
    pages            = {1-12},
    publisher        = {MDPI},
    doi              = {10.3390/bioengineering4020038},
}


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