Engineering of CHO Cells for the Production of Recombinant Glycoprotein Vaccines with Xylosylated N-glycans
Title:
Engineering of CHO Cells for the Production of Recombinant Glycoprotein Vaccines with Xylosylated N-glycans
Authors:
Abstract:
Xylose is a general component of O-glycans in mammals. Core-xylosylation of N-glycans is only found in plants and helminth. Consequently, xylosylated N-glycans cause immunological response in humans. We have used the F-protein of the human respiratory syncytial virus (RSV), one of the main causes of respiratory tract infection in infants and elderly, as a model protein for vaccination. The RSV-F protein was expressed in CHO-DG44 cells, which were further modified by co-expression of β1,2-xylosyltransferase from Nicotiana tabacum. Xylosylation of RSV-F N-glycans was shown by monosaccharide analysis and MALDI-TOF mass spectrometry. In immunogenic studies with a human artificial lymph node model, the engineered RSV-F protein revealed improved vaccination efficacy.
Year:
2017
Publication type:
Journal article
Journal:
Bioengineering
ISSN:
2306-5354
Number:
2
Volume:
4
Pages:
1-12
Language:
English
Document status:
Open access
PubListerURL:
https://publister.bib.th-wildau.de/publister/public/publication/2251
Keywords:
CHO; glycoengineering; respiratory syncytial virus; vaccine; xylose
Sandig, G., von Horsten, H., Radke, L., Blanchard, V., Frohme, M., Giese, C., et al. (2017). Engineering of CHO Cells for the Production of Recombinant Glycoprotein Vaccines with Xylosylated N-glycans Bioengineering. 4 (2), 1-12.
@article{2251,
author = {Sandig, Grit and von Horsten, Hans Henning and Radke, Lars and Blanchard, Véronique and Frohme, Marcus and Giese, Christoph and Sandig, Volker and Hinderlich, Stephan},
title = {Engineering of CHO Cells for the Production of Recombinant Glycoprotein Vaccines with Xylosylated N-glycans},
journal = {Bioengineering},
year = {2017},
volume = {4},
number = {2},
pages = {1-12},
publisher = {MDPI},
doi = {10.3390/bioengineering4020038},
}