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Electroactive Multilayer Assemblies of Bilirubin Oxidase and Human Cytochrome C Mutants: Insight in Formation and Kinetic Behavior

Title:
Electroactive Multilayer Assemblies of Bilirubin Oxidase and Human Cytochrome C Mutants: Insight in Formation and Kinetic Behavior
Abstract:
Here, we report on cytochrome c/bilirubin oxidase multilayer electrodes with different cytochrome c (cyt c) forms including mutant forms of human cyt c, which exhibit different reaction rates with bilirubin oxidase (BOD) in solution. The multilayer formation via the layer-by-layer technique and the kinetic behavior of the mono (only cyt c) and biprotein (cyt c and BOD) multilayer systems are studied by SPR and cyclic voltammetry. For the layer construction, sulfonated polyaniline is used. The only cyt c containing multilayer electrodes show that the quantity of deposited protein and the kinetic behavior depend on the cyt c form incorporated. In the case of the biprotein multilayer with BOD, it is demonstrated that the catalytic signal chain from the electrode via cyt c to BOD and oxygen can be established with all chosen cyt c forms. However, the magnitude of the catalytic current as well as the kinetic behavior differ significantly. We conclude that the different cytochrome c forms affect three parameters, identified here, to be important for the functionality of the multilayer system: the amount of molecules per layer, which can be immobilized on the electrodes, the cyt c self-exchange rate, and the rate constant for the reaction with BOD.
Year:
2011
Publication type:
Journal article
Journal:
Langmuir
ISSN:
0743-7463
Number:
7
Volume:
27
Pages:
4202-4211
Language:
English
Document status:
Published
PubListerURL:
https://publister.bib.th-wildau.de/publister/public/publication/231
Wegerich, F., Turano, P., Allegrozzi, M., Möhwald, H., & Lisdat, F. (2011). Electroactive Multilayer Assemblies of Bilirubin Oxidase and Human Cytochrome C Mutants: Insight in Formation and Kinetic Behavior Langmuir. 27 (7), 4202-4211.
@article{231,
    author           = {Wegerich, Franziska and Turano, Paola and Allegrozzi, Marco and Möhwald, Helmuth and Lisdat, Fred},
    title            = {Electroactive Multilayer Assemblies of Bilirubin Oxidase and Human Cytochrome C Mutants: Insight in Formation and Kinetic Behavior},
    journal          = {Langmuir},
    year             = {2011},
    volume           = {27},
    number           = {7},
    pages            = {4202-4211},
    doi              = {10.1021/la104964z},
}


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