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Pushing the detection limits: the evanescent field in surface plasmon resonance and analyte-induced folding observation of long human telomeric repeats

Title:
Pushing the detection limits: the evanescent field in surface plasmon resonance and analyte-induced folding observation of long human telomeric repeats
Abstract:
Conventional analysis of molecular interactions by surface plasmon resonance is achieved by the observation of optical density changes due to analyte binding to the ligand on the surface. Low molecular weight interaction partners are normally not detected. However, if a macromolecule such as DNA can extend beyond the evanescent field and analyte interaction results in a large-scale contraction, then the refractive index changes due to the increasing amount of macromolecules close to the surface. In our proof-of-principle experiment we could observe the direct folding of long, human telomeric repeats induced by the small analyte potassium using surface plasmon resonance spectroscopy. This work demonstrates the feasibility of new evanescent field-based biosensors that can specifically observe small molecule interactions. Highlights ► We have detected the folding of long telomeres in response to potassium ions by SPR. ► Telomere contraction leads to refractive index changes within the evanescent field. ► This new detection principle can be used for the measurement of small analytes.
Year:
2012
Publication type:
Journal article
Journal:
Biosensors and Bioelectronics
ISSN:
0956-5663
Number:
1
Volume:
31
Pages:
571-574
Language:
English
Document status:
Published
PubListerURL:
https://publister.bib.th-wildau.de/publister/public/publication/2534
Keywords:
Evanescent field; Surface plasmon resonance; Long concatemeric repeat; Human telomere; Ligand-induced folding
Schlachter, C., Lisdat, F., Frohme, M., Erdmann, V., Konthur, Z., Lehrach, H., et al. (2012). Pushing the detection limits: the evanescent field in surface plasmon resonance and analyte-induced folding observation of long human telomeric repeats Biosensors and Bioelectronics. 31 (1), 571-574.
@article{2534,
    author           = {Schlachter, Constanze and Lisdat, Fred and Frohme, Marcus and Erdmann, Volker A. and Konthur, Zoltán and Lehrach, Hans and Glökler, Jörn Felix},
    title            = {Pushing the detection limits: the evanescent field in surface plasmon resonance and analyte-induced folding observation of long human telomeric repeats},
    journal          = {Biosensors and Bioelectronics},
    year             = {2012},
    volume           = {31},
    number           = {1},
    pages            = {571-574},
    doi              = {10.1016/j.bios.2011.11.003},
    url              = {https://www.sciencedirect.com/science/article/pii/S0956566311007470},
}


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