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Toxicity of C60 fullerene–cisplatin nanocomplex against Lewis lung carcinoma cells

Title:
Toxicity of C60 fullerene–cisplatin nanocomplex against Lewis lung carcinoma cells
Abstract:
Cisplatin (Cis-Pt) is the cytotoxic agent widely used against tumors of various origin, but its therapeutic efficiency is substantially limited by a non-selective effect and high toxicity. Conjugation of Cis-Pt with nanocarriers is thought to be one option to enable drug targeting. The aim of this study was to estimate toxic effects of the nanocomplex formed by noncovalent interaction of C60 fullerene with Cis-Pt against Lewis lung carcinoma (LLC) cells in comparison with free drug. Scanning tunneling microscopy showed that the minimum size of C60–Cis-Pt nanoparticles in aqueous colloid solution was 1.1 nm whereas that of C60 fullerene was 0.72 nm, thus confirming formation of the nanocomplex. The cytotoxic effect of C60–Cis-Pt nanocomplex against LLC cells was shown to be higher with IC50 values 3.3 and 4.5 times lower at 48 h and 72 h, respectively, as compared to the free drug. 12.5 µM Cis-Pt had no effect on LLC cell viability and morphology while C60–Cis-Pt nanocomplex in Cis-Pt-equivalent concentration substantially decreased the cell viability, impaired their shape and adhesion, inhibited migration and induced accumulation in proapoptotic subG1 phase. Apoptosis induced by the C60–Cis-Pt nanocomplex was confirmed by caspase 3/7 activation and externalization of phosphatidylserine on the outer surface of LLC cells with the double Annexin V-FITC/PI staining. We assume that C60 fullerene as a component of the C60–Cis-Pt nanocomplex promoted Cis-Pt entry and intracellular accumulation thus contributing to intensification of the drug’s toxic effect against lung cancer cells.
Year:
2019
Publication type:
Journal article
Journal:
Archives of Toxicology
ISSN:
1432-0738
Number:
5
Volume:
93
Pages:
12131226
Language:
English
Document status:
Published
PubListerURL:
https://publister.bib.th-wildau.de/publister/public/publication/2695
Prylutska, S., Grynyuk, I., Skaterna, T., Horak, I., Grebinyk, A., Drobot, L., et al. (2019). Toxicity of C60 fullerene–cisplatin nanocomplex against Lewis lung carcinoma cells Archives of Toxicology. 93 (5), 12131226.
@article{2695,
    author           = {Prylutska, Svitlana and Grynyuk, I. and Skaterna, Tetiana and Horak, Iryna and Grebinyk, Anna and Drobot, Liudmyla and Matyshevska, Olga and Senenko, Anton and Prylutskyy, Yuriy and Naumovets, Anton and Ritter, Uwe and Frohme, Marcus},
    title            = {Toxicity of C60 fullerene–cisplatin nanocomplex against Lewis lung carcinoma cells},
    journal          = {Archives of Toxicology},
    year             = {2019},
    volume           = {93},
    number           = {5},
    pages            = {12131226},
    publisher        = {Springer Nature},
    doi              = {10.1007/s00204-019-02441-6},
}


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