Hinweis (Dez. 2019): PubLister wird demnächst abgelöst. Publikationen der Technischen Hochschule Wildau werden zukünftig in HISinOne nachgewiesen. In diesem Zusammenhang kann es momentan zu Überarbeitungen und zu temporären Veränderungen Ihrer persönlichen Publikationslisten kommen. Bei weiteren Fragen wenden Sie sich bitte an friederike.borchert [at] th-wildau [dot] de.

Multiplexed Readout of Enzymatic Reactions by Means of Laterally Resolved Illumination of Quantum Dot Electrodes

Title:
Multiplexed Readout of Enzymatic Reactions by Means of Laterally Resolved Illumination of Quantum Dot Electrodes
Abstract:
Triggering electrochemical reactions with light provides a powerful tool for the control of complex reaction schemes on photoactive electrodes. Here, we report on the light-directed, multiplexed detection of enzymatic substrates using a nonstructured gold electrode modified with CdSe/ZnS quantum dots (QDs) and two enzymes, glucose oxidase (GOx) and sarcosine oxidase (SOx). While QDs introduce visible-light sensitivity into the electrode architecture, GOx and SOx allow for a selective conversion of glucose and sarcosine, respectively. For the QD immobilization to the gold electrode, a linker-assisted approach using trans-4,4′-stilbenedithiol has been used, resulting in the generation of a photocurrent. Subsequently, GOx and SOx have been immobilized in spatially separated spots onto the QD electrode. For the local readout of the QD electrode, a new measurement setup has been developed by moving a laser pointer across the surface to defined positions on the chip surface. The amplitudes of the photocurrents upon illumination of the GOx or SOx spot depend in a concentration-dependent manner on the presence of glucose and sarcosine, respectively. This measurement also allows for a selective detection in the presence of other substances. The setup demonstrates the feasibility of multiplexed measurements of enzymatic reactions using a focused light pointer, resulting in an illumination area with a diameter of 0.3 mm for analyzing spots of different enzymes. Moving the laser pointer in the x- and y-direction and simultaneously detecting the local photocurrent also allow a spatial imaging of enzyme immobilization. Here, not only the spot dimensions but also the activity of the enzyme can be verified.
Year:
2019
Publication type:
Journal article
Journal:
ACS Applied Materials & Interfaces
ISSN:
1944-8252
Number:
24
Volume:
11
Pages:
21830-21839
Language:
English
Document status:
Published
PubListerURL:
https://publister.bib.th-wildau.de/publister/public/publication/2711
Keywords:
photoelectrochemistry glucose oxidase sarcosine oxidase quantum dots multiplexed detection imaging
Zhao, S., Völkner, J., Riedel, M., Witte, G., Zhao, Y., Lisdat, F., et al. (2019). Multiplexed Readout of Enzymatic Reactions by Means of Laterally Resolved Illumination of Quantum Dot Electrodes ACS Applied Materials & Interfaces. 11 (24), 21830-21839.
@article{2711,
    author           = {Zhao, Shuang and Völkner, Johannes and Riedel, Marc and Witte, Gregor and Zhao, Yue and Lisdat, Fred and Parak, Wolfgang J.},
    title            = {Multiplexed Readout of Enzymatic Reactions by Means of Laterally Resolved Illumination of Quantum Dot Electrodes},
    journal          = {ACS Applied Materials & Interfaces},
    year             = {2019},
    volume           = {11},
    number           = {24},
    pages            = {21830-21839},
    publisher        = {American Chemical Society (ACS)},
    doi              = {10.1021/acsami.9b03990},
}


Search this title in Google Scholar