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A Novel Optical Method To Reversibly Control Enzymatic Activity Based On Photoacids

Title:
A Novel Optical Method To Reversibly Control Enzymatic Activity Based On Photoacids
Abstract:
Most biochemical reactions depend on the pH value of the aqueous environment and some are strongly favoured to occur in an acidic environment. A non-invasive control of pH to tightly regulate such reactions with defined start and end points is a highly desirable feature in certain applications, but has proven difficult to achieve so far. We report a novel optical approach to reversibly control a typical biochemical reaction by changing the pH and using acid phosphatase as a model enzyme. The reversible photoacid G-acid functions as a proton donor, changing the pH rapidly and reversibly by using high power UV LEDs as an illumination source in our experimental setup. The reaction can be tightly controlled by simply switching the light on and off and should be applicable to a wide range of other enzymatic reactions, thus enabling miniaturization and parallelization through non-invasive optical means.
Year:
2019
Publication type:
Journal article
Journal:
Scientific Reports
ISSN:
2045-2322
Volume:
9
Pages:
14372 (2019)
Language:
English
Document status:
Open access
PubListerURL:
https://publister.bib.th-wildau.de/publister/public/publication/2748
Kagel, H., Bier, F., Frohme, M., & Glökler, J. (2019). A Novel Optical Method To Reversibly Control Enzymatic Activity Based On Photoacids Scientific Reports. 9, 14372 (2019).
@article{2748,
    author           = {Kagel, Heike and Bier, Frank F. and Frohme, Marcus and Glökler, Jörn Felix},
    title            = {A Novel Optical Method To Reversibly Control Enzymatic Activity Based On Photoacids},
    journal          = {Scientific Reports},
    year             = {2019},
    volume           = {9},
    pages            = {14372 (2019)},
    publisher        = {Springer Nature},
    doi              = {10.1038/s41598-019-50867-w},
}


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