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Development of New Microsatellite (STR) Markers for Montagu’s Harrier (Circus pygargus) via 454 Shot-Gun Pyrosequencing

Title:
Development of New Microsatellite (STR) Markers for Montagu’s Harrier (Circus pygargus) via 454 Shot-Gun Pyrosequencing
Abstract:
During the last decades the ground-breeding Montagu’s harrier (Circus pygargus, Linnaeus, 1758) has changed its breeding habitats in Europe to agricultural areas in which many local populations would be close to extinction without a special nest protection regime. Although Montagu’s harrier is a well-studied species in terms of ecology and breeding biology, its genetic structure and population genetics are almost unknown. As there is a lack of good genetic markers we developed a set of 19 microsatellite markers comprising 16 new STR markers which were identified by next-generation sequencing (NGS) using 454 shot-gun pyrosequencing of genomic DNA. The STR markers were arranged into three multiplex PCR sets for high throughput genotyping and characterised. The marker set provides a powerful tool for kinship analysis. The combined non-exclusion probability for parent pairs was 1.13*10-11. Only three loci showed PIC values < 0.50. In total, 121 known family relationships were compared with genetically calculated ones to test the markers suitability for parentage analysis. In 97.5% of all cases full-sibships were accurately determined and 97.6% of all mothers were assigned correctly to their chicks. The present multiplex PCR panels can be used to investigate several hypotheses concerning breeding behaviour, kinship, exchange rates between populations and phylogeography
Year:
2014
Publication type:
Journal article
Journal:
The Open Ornithology Journal
ISSN:
1874-4532
Volume:
7
Pages:
11-18
Language:
English
Document status:
Open access
PubListerURL:
https://publister.bib.th-wildau.de/publister/public/publication/402
Keywords:
Circus pygargus, Dipetalogaster maxima, genotyping, microsatellites, Montagu’s harrier, multiplex PCR, next-generation sequencing, parentage analysis
Janowski, S., Grohme, M., Frohme, M., & Wink, M. (2014). Development of New Microsatellite (STR) Markers for Montagu’s Harrier (Circus pygargus) via 454 Shot-Gun Pyrosequencing The Open Ornithology Journal. 7, 11-18.
@article{402,
    author           = {Janowski, Susann and Grohme, Markus Alexander and Frohme, Marcus and Wink, Michael},
    title            = {Development of New Microsatellite (STR) Markers for Montagu’s Harrier (Circus pygargus) via 454 Shot-Gun Pyrosequencing},
    journal          = {The Open Ornithology Journal},
    year             = {2014},
    volume           = {7},
    pages            = {11-18},
    doi              = {10.2174/1874453201407010011},
}


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