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Superoxide Biosensing with Engineered Cytochrome c

Title:
Superoxide Biosensing with Engineered Cytochrome c
Abstract:
Several mutation positions have been chosen for introducing positively charged lysines in human cytochrome c (cyt c) with the aim of increasing the reaction rate with superoxide radicals (SO) and thus, the sensitivity of an electrochemical cyt c based SO biosensor. The impact of the mutations on structural and redox properties as well as on the reaction rate with SO are verified. Four mutants show a higher reaction rate with the radical compared to the wild type. These mutants are used for the construction of SO sensors based on thiol-modified gold electrodes and covalently fixed proteins. The E66K mutant electrode has a clearly higher sensitivity in comparison to the wildtype based sensor.
Year:
2009
Publication type:
Journal article
Journal:
Procedia Chemistry
ISSN:
1876-6196
Number:
1
Volume:
1
Pages:
1287–1290
Language:
English
Document status:
Open access
PubListerURL:
https://publister.bib.th-wildau.de/publister/public/publication/448
Keywords:
biosensor; cytochrome c, superoxide, protein engineering
Wegerich, F., Turano, P., Allegrozzi, M., Möhwald, H., & Lisdat, F. (2009). Superoxide Biosensing with Engineered Cytochrome c Procedia Chemistry. 1 (1), 1287–1290.
@article{448,
    author           = {Wegerich, Franziska and Turano, Paola and Allegrozzi, Marco and Möhwald, Helmuth and Lisdat, Fred},
    title            = {Superoxide Biosensing with Engineered Cytochrome c},
    journal          = {Procedia Chemistry},
    year             = {2009},
    volume           = {1},
    number           = {1},
    pages            = {1287–1290},
    doi              = {10.1016/j.proche.2009.07.321},
}


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