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Investigating heat shock proteins of tardigrades in active versus anhydrobiotic state using shotgun proteomics

Title:
Investigating heat shock proteins of tardigrades in active versus anhydrobiotic state using shotgun proteomics
Abstract:
The eutardigrade Milnesium tardigradum can undergo cryptobiosis and can survive extreme environmental conditions. However, the survival mechanisms of tardigrades are still poorly understood. Heat shock proteins (Hsps) as an important subgroup of chaperones which protect proteins from irreversible aggregation and degradation might play an important role in anhydrobiosis. In this report, we therefore investigated Hsps in tardigrades in the active versus the anhydrobiotic state employing proteomics techniques. Protein lysates from tardigrades in both states were separated by one-dimensional gel electrophoresis, in-gel digested with trypsin and tryptic peptides were analyzed by high sensitive nanoLC-ESI-MS/MS on a LTQ-Orbitrap mass spectrometer. This study indicates the presence of Hsps of the major chaperone families Hsp40, Hsp60, Hsp70, Hsp90, small Hsps and furthermore nucleotide exchange factors and co-chaperones in Milnesium tardigradum. A comparative analysis of the identified Hsps in both states was performed by calculating the exponentially modified protein abundance index
Year:
2011
Publication type:
Journal article
Journal:
Journal of Zoological Systematics and Evolutionary Research
ISSN:
1439-0469
Number:
Supplement s1
Volume:
49
Pages:
111 - 119
Language:
English
Document status:
Published
PubListerURL:
https://publister.bib.th-wildau.de/publister/public/publication/452
Keywords:
Tardigrades; heat shock proteins; Hsp60 (chaperonin); Hsp40 (DnaJ); Hsp70 (DnaK); Hsp90; small heat shock proteins; nucleotide exchange factors; co-chaperones; high throughput proteomics
Schokraie, E., Hotz-Wagenblatt, A., Warnken, U., Frohme, M., Dandekar, T., & Schill, R. (2011). Investigating heat shock proteins of tardigrades in active versus anhydrobiotic state using shotgun proteomics Journal of Zoological Systematics and Evolutionary Research. 49 (Supplement s1), 111 - 119.
@article{452,
    author           = {Schokraie, Elham and Hotz-Wagenblatt, Agnes and Warnken, Uwe and Frohme, Marcus and Dandekar, Thomas and Schill, Ralph O.},
    title            = {Investigating heat shock proteins of tardigrades in active versus anhydrobiotic state using shotgun proteomics},
    journal          = {Journal of Zoological Systematics and Evolutionary Research},
    year             = {2011},
    volume           = {49},
    number           = {Supplement s1},
    pages            = {111 - 119},
    doi              = {10.1111/j.1439-0469.2010.00608.x },
}


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