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Intact cell mass spectrometry as a rapid and specific tool for the differentiation of toxic effects in cell-based ecotoxicological test systems

Title:
Intact cell mass spectrometry as a rapid and specific tool for the differentiation of toxic effects in cell-based ecotoxicological test systems
Abstract:
In the last few decades, MALDI-TOF MS has become a useful technique not only in proteomics, but also as a fast and specific tool for whole cell analysis through intact cell mass spectrometry (IC-MS). The present study evaluated IC-MS as a novel tool for the detection of distinct patterns that can be observed after exposure to a certain toxin or concentration by utilizing the eukaryotic fish cell line RTL-W1. Two different viability assays were performed to define the range for IC-MS investigations, each of which employing copper sulfate, acridine, and β-naphthoflavone (BNF) as model compounds for several classes of environmental toxins. The IC-MS of RTL-W1 cells revealed not only specific spectral patterns for the various toxins, but also that the concentration used had an effect on RTL-W1 profiles. After the exposure with copper sulfate and acridine, the spectra of RTL-W1 showed a significant increase of certain peaks in the higher mass range (m/z >7000), which is probably attributed to the apoptosis of RTL-W1. On the contrary, exposure to BNF showed a distinct change of ion abundances only in the lower mass range (m/z <7000). Furthermore, a set of mass peaks could be identified as a specific biomarker for a single toxin treatment, so IC-MS demonstrates a new method for the distinction of toxic effects in fish cells. Due to fast sample preparation and high throughput, IC-MS offers great potential for ecotoxicological studies to investigate cellular effects of different substances and complex environmental samples.
Year:
2015
Publication type:
Journal article
Journal:
Analytical and Bioanalytical Chemistry
ISSN:
1618-2642
Number:
25
Volume:
407
Pages:
7721–7731
Language:
English
Document status:
Open access
PubListerURL:
https://publister.bib.th-wildau.de/publister/public/publication/914
Keywords:
RTL-W1, Intact cell mass spectrometry, MALDI, Acridine, β-Naphthoflavone, Copper sulfate
Kober, L., Meyer-Alert, H., Grienitz, D., Hollert, H., & Frohme, M. (2015). Intact cell mass spectrometry as a rapid and specific tool for the differentiation of toxic effects in cell-based ecotoxicological test systems Analytical and Bioanalytical Chemistry. 407 (25), 7721–7731.
@article{914,
    author           = {Kober, Liane and Meyer-Alert, Henriette and Grienitz, Desirée and Hollert, Henner and Frohme, Marcus},
    title            = {Intact cell mass spectrometry as a rapid and specific tool for the differentiation of toxic effects in cell-based ecotoxicological test systems},
    journal          = {Analytical and Bioanalytical Chemistry},
    year             = {2015},
    volume           = {407},
    number           = {25},
    pages            = { 7721–7731},
    publisher        = {Springer},
    doi              = {10.1007/s00216-015-8937-2},
    url              = {http://link.springer.com/article/10.1007/s00216-015-8937-2},
}


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