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Robust gene expression and mutation analyses of RNA-sequencing of formalin-fixed diagnostic tumor samples

Title:
Robust gene expression and mutation analyses of RNA-sequencing of formalin-fixed diagnostic tumor samples
Abstract:
Current genomic studies are limited by the availability of fresh tissue samples. Here, we show that Illumina RNA sequencing of formalin-fixed diagnostic tumor samples produces gene expression that is strongly correlated with matched frozen tumor samples (r > 0.89). In addition, sequence variations identified from FFPE RNA show 99.67% concordance with that from exome sequencing of matched frozen tumor samples. Because FFPE is a routine diagnostic sample preparation, the feasibility results reported here will facilitate the setup of large-scale research and clinical studies in medical genomics that are currently limited by the availability of fresh frozen samples.
Year:
2015
Publication type:
Journal article
Journal:
Scientific reports
ISSN:
2045-2322
Volume:
5
Pages:
Artikel 12335
Language:
English
Document status:
Open access
PubListerURL:
https://publister.bib.th-wildau.de/publister/public/publication/939
Keywords:
Chronic Lymphocytic-Leukemia; Somatic Mutations; Seq Data; Cancer; Tissues; Identification; Alignment; Genomes
Graw, S., Meier, R., Minn, K., Bloomer, C., Godwin, A., Fridley, B., et al. (2015). Robust gene expression and mutation analyses of RNA-sequencing of formalin-fixed diagnostic tumor samples Scientific reports. 5, Artikel 12335.
@article{939,
    author           = {Graw, Stefan and Meier, Richard and Minn, Kay and Bloomer, Clark and Godwin, Andrew K. and Fridley, Brooke and Vlad, Anda and Beyerlein, Peter and Chien, Jeremy},
    title            = {Robust gene expression and mutation analyses of RNA-sequencing of formalin-fixed diagnostic tumor samples},
    journal          = {Scientific reports},
    year             = {2015},
    volume           = {5},
    pages            = {Artikel 12335},
    publisher        = {Springer Nature},
    doi              = {10.1038/srep12335},
}


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