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Prediction of cytokine release using gene expression profile analysis

Title:
Prediction of cytokine release using gene expression profile analysis
Abstract:
Cytokines are signal transmitters in complex regulatory networks, which modify the behaviour of cells of the immune system. They are used to monitor the immunological reaction to various agents. With the adequate knowledge of cytokine expression kinetics one could circumvent the need for repeated protein measurements. We correlate here the release of four key cytokines on mRNA and protein level and present methods for quality control and save costs in an academic laboratory. Gene expression of stimulated cells was analyzed with RT-PCR and cytokine concentrations in supernatants of PBMC cell culture were determined in multiplex with a bead-based suspension-array system. mRNA expression was shown to have a predictive quality for cytokine release.
Year:
2010
Publication type:
Journal article
Journal:
Chimica oggi : mensile di ricerca e tecnologia applicata
ISSN:
0392-839X
Number:
5
Volume:
28
Pages:
1-3
Language:
English
Document status:
Published
PubListerURL:
https://publister.bib.th-wildau.de/publister/public/publication/989
Radke, L., López, D., Lubitz, A., Giese, C., & Frohme, M. (2010). Prediction of cytokine release using gene expression profile analysis Chimica oggi : mensile di ricerca e tecnologia applicata. 28 (5), 1-3.
@article{989,
    author           = {Radke, Lars and López, Diana A. and Lubitz, Annika and Giese, Christoph and Frohme, Marcus},
    title            = {Prediction of cytokine release using gene expression profile analysis},
    journal          = {Chimica oggi : mensile di ricerca e tecnologia applicata},
    year             = {2010},
    volume           = {28},
    number           = {5},
    pages            = {1-3},
}


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